Timely diagnosis of cancer,
by detection and capturing of circulating cancer or endothelial progenitor cells, using specific markers and devices, is important for the promising prognosis and for the successful treatment
However, there are combinations of markers that are very specific to one particular cell, e.g. cancer cell, or stem cell.
We propose a microchannel-based device that is able to capture a specific cell type using combined markers for the analysis of cellular content of samples. The device is essentially a set of narrow channels through which the cells can be pumped through. Some areas on channel wall are functionalized with a combination of receptors that are binding to their corresponding markers on the cell’s surface.
The flow conditions and the channel dimensions are optimized on the way that if more than one marker is present on the cell surface then the cell gets stuck in the channel.
The number of such stuck channels can be determined by optical or electrical means, e.g. using the change in the impedance of the channel.
Patent no.: Ep14168842 “Microchannel-based cell capture device for the analysis of cellular content of samples”
This process can be achieved through the incorporation of endothelial progenitor cells (EPCs) into the endothelial tube of novel blood capillaries.
Based on several observations, the circulating levels of these cells are associated with the severity of the given disease. In line with that, enumeration of EPCs can help to assess disease progression, prognosis and therapeutic efficacy.
This angiogenesis related project has been successfully completed laying down the principles of developing a detection method for enumeration circulating EPCs.
Grant: INNO_08_KM-KLABIN08 (INNOCSEKK_PLUSZ) (NKFIH – ETH Zürich)