Services
iN VItrO
KINETO Lab offers and provides wide range of in vitro preclinical services on various tumor models which allow you to design the most suitable study to determine the activity of your compound, select the best compound and prove the hypothesis of your research.
For the development of your therapeutics, determination of its mechanisms of action, or characterization of samples, we can provide the following methods with analysis and reports:
Cytotoxicity and antiproliferative assays
Molecular biology assays
CYTOTOXICITY AND ANTIPROLIFERATIVE ASSAYS DETERMIning COMPOUND EFFICACY
At KINETO Lab, we perform different assays providing the most suitable method to assess and to determine the cytotoxic and anti-proliferative potential of your compounds on chosen tumor models using a variety of cell viability assays full analysis included.
Testing on panels of cell lines
Your compounds can be tested on panel of cell lines with different target expression level to estimate their selectivity towards the target, while the screening of your compounds on non-cancerous cell lines provides you possibility to estimate selectivity.
Interaction of multiple compounds, coculture of multiple cell lines also fit our profile providing you the most detailed in vitro characterization.
70 human and 10 murine cell lines
For the studies, we have knowledge and experience working with over 70 human cell lines and 10 murine cell lines of cancerous or non-cancerous tissue origin to test your compounds.
List of cell line models
Various methods
Our lab is routinely using various assays such as:
MTT
Sulphorhodamine B
PrestoBlue
Luminescent ATP-measurement
Fluorescent cell line-based viability and proliferation measurements
3D spheroid assays
We can compare multiple methods to establish the optimal measurement system for your needs.
Hypoxic conditions
Hypoxia has a pivotal role in tumor progression and it was shown to correlate with poor prognosis of cancer patients, leading to therapy resistance.
KINETO Lab has suitable experience, equipments and models to study hypoxia mechanisms as well as to investigate efficacy of therapeutics in this condition.
For further details regarding services, please contact us on info@kinetolab.hu
