KINETO Lab offers and provides wide range of in vitro preclinical services on various tumor models which allow you to design the most suitable study to determine activity of your compound, select lead compound and prove a concept of your research.
For the development of your therapeutics, determining its mechanisms of action, or characterization of samples, we can provide the following methods with analysis and reports:
Cytotoxicity and antiproliferative assays for determination compound efficacy
At KINETO Lab, we perform different assays providing the most suitable method to assess and to determine the cytotoxic and anti-proliferative potential of your compounds on chosen tumor models using a variety of cell viability assays full analysis included.
Your compounds can be tested on panel of cell lines with different target expression level to estimate their selectivity towards the target, while the screening of your compounds on non-cancerous cell lines provides you possibility to estimate selectivity. Interaction of multiple compounds, coculture of multiple cell lines also fit our profile providing you the most detailed in vitro characterization. For the studies, we have a collection of over 70 human cell lines and 10 murine cell lines of cancerous or non-cancerous tissue origin to test your compounds.
Molecular Biology assays
- Western blot for protein quantification
- Rt-PCR for RNA quantification
- DNA and RNA isolation and characterization (RFLP, Sanger sequencing)
- allele-specific PCR
- flow cytometry
- Immunocytochemistry (fluorescence, confocal and video microscopy)
- 3D spheroid assays
- Adhesion assay
- Invasion/migration assays
- 2D: cell culture wounding, phagokinetic track assay, video microscopy, emigration of cells from tumor spheroids (scatter).
- 3D: Boyden chamber (cell number is determined on the lower side of a pored membrane), migration in extracellular matrix gels (collagen, fibrin, basement membrane), organ cultures (chorioallantoic membrane, denuded cornea).
- Cellular uptake assays (flow cytometry)
- Cell motility assays (video microscopy)
- Intravasation and extravasation models