For immuno-oncology research, KINETO Lab established in vitro models such as:

• Antibody-dependent cellular cytotoxicity (ADCC) assays with fresh immune cells where an immune mechanism through which Fc receptor-bearing effector cells, such as Natural Killer (NK) lymphocytes, can recognize, bind and kill antibody-coated target cells expressing tumor- or pathogen-derived antigens on their surface
• Coculture with immune cells
• Antibody attachment methods for flow cytometry to show whether your compound helps or inhibits antibody binding

We use in vivo models too, in order to study immunology aspects of oncology:

• Mouse-on-mouse (allograft) models, for the investigation of the complete murine immune system targets
• For human specific targets we can map NK cell and macrophage activity in the strains of mice with different level of immune system suppression (SCID has both NK and macrophage activity, NOD-SCID has only functional macrophages, while NSG lacks both). Thus, immune cell-related reactions can be identified
• Also, NK inhibitor or macrophage inhibitor could be applied in order to check their influence on drug activity